This web page was produced as an assignment for Genetics 564, an undergraduate capstone course at UW-Madison
GEO Profiles
GEO Profiles is a database that collects gene expression profiles that are obtained from curated GEO DataSets. This data is presented in a chart and shows the expression level of genes in an experiment. [1]
This chart displayed to the left shows the expression level when peripheral blood mononuclear cells are exposed to benzene. With the exposure of benzene the expression levels tends to go up. [2] |
GEO DataSet
GEO DataSets collects original sumitted records from research completed. Curated DataSets have helpful analysis features to identify differences in gene expression levels and cluster heatmaps. [3]
This heat map displayed to the right shows the analysis of peripheral blood mononuclear cells from workers at a shoe factory that were exposed to benzene, a leukemogen. This study gives insight into the mechanism behind benzene carcinogenicity. [4] |
Analysis
Gene expression data can be a great tool to discover what pathways a gene may be in. They can be used to compare the global gene expression changes in mutant/diseased individuals compared to wildtype/healthy individuals. This can give insight into potential reasons for diseases and eventually therapeutic treatments. As seen in the GEO profile, benzene creates a change in expression of MITF. In a case where there is down regulation of a gene, a drug could possibly be used to up-regulate it instead. There is a potential that a drug up-regulates gene expression, as seen in the GEO DataSet, as could cause a disease. Therefore, research would need to be done to counteract these affects.
References
1. About GEO Profiles - GEO - NCBI. (n.d.). Retrieved March 08, 2017, from https://www.ncbi.nlm.nih.gov/geo/info/profiles.html
2. MITF - Occupational benzene exposure: peripheral blood mononuclear cells (HG-U133B). (n.d.). Retrieved March 08, 2017, from https://www.ncbi.nlm.nih.gov/geoprofiles/60914459
3. About GEO DataSets - GEO - NCBI. (n.d.). Retrieved March 09, 2017, from https://www.ncbi.nlm.nih.gov/geo/info/datasets.html
4. Occupational benzene exposure: peripheral blood mononuclear cells (HG-U133B). (n.d.). Retrieved March 09, 2017, from https://www.ncbi.nlm.nih.gov/gds?LinkName=geoprofiles_gds&from_uid=60914459
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1. About GEO Profiles - GEO - NCBI. (n.d.). Retrieved March 08, 2017, from https://www.ncbi.nlm.nih.gov/geo/info/profiles.html
2. MITF - Occupational benzene exposure: peripheral blood mononuclear cells (HG-U133B). (n.d.). Retrieved March 08, 2017, from https://www.ncbi.nlm.nih.gov/geoprofiles/60914459
3. About GEO DataSets - GEO - NCBI. (n.d.). Retrieved March 09, 2017, from https://www.ncbi.nlm.nih.gov/geo/info/datasets.html
4. Occupational benzene exposure: peripheral blood mononuclear cells (HG-U133B). (n.d.). Retrieved March 09, 2017, from https://www.ncbi.nlm.nih.gov/gds?LinkName=geoprofiles_gds&from_uid=60914459
Images
Banner: http://www.africapublic.com/girl-with-ocean-eyes/